DNA Methylation Sequencing

What Is DNA Methylation Sequencing?

DNA methylation is important epigenetic marker information, and obtaining genome-wide methylation level data for all C sites is important for epigenetic spatiotemporal specificity studies. There are various types of methylation modifications, among which the most common and widely studied is the methylation modification at position 5 of the cytosine heterocycle, also known as 5-methylcytosine (5mC).

DNA methylation can cause changes in chromatin structure, DNA conformation, DNA stability and the way DNA interacts with proteins, thereby controlling gene expression. Abnormal DNA methylation can lead to developmental abnormalities, tumors, and other diseases. Therefore, the study of DNA methylation is of great importance for an in-depth understanding of gene expression, individual development, and the mechanisms of disease onset and development.

Methylation sequencing is a low-cost, high-efficiency and high-accuracy mapping of DNA methylation levels based on a new generation high-throughput sequencing platform, combined with genome-wide data processing and bioinformatics data analysis technologies. DNA methylation is important epigenetic marker information, and obtaining methylation level data of all C loci in the whole genome range is important for epigenetic Spatio-temporal specificity study. In terms of years' professional experience in this field, Creative Biogene can provide you with the most affordable and high-quality DNA methylation sequencing services.

The process of DNA methylation sequencing.Figure 1. The process of DNA methylation sequencing.

Advantages of Our DNA Methylation Sequencing

  • Highly flexible
    It can directly sequence hypermethylated fragments of any species without known genomic sequence information.
  • Wide detection range
    It covers the entire genome-wide methylation region.
  • High accuracy
    It is capable of precise localization within 50 bases of the actual binding site.
  • Digital signal
    Direct sequencing and quantification of methylated fragments without cross-reactivity and background noise associated with the fluorescent analog signal of traditional microarray hybridization.

Sample Requirements

  • Sample types
    Cell, tissue, body fluid, total gDNA or post-IP DNA. Other types of samples please ask for details.
  • Sample volume
    • Cells 5×108
    • Tissue 100 mg
    • Post-IP DNA 10 ng
    • gDNA 3μg
  • Sample transport and storage
    • Sample transportation: samples are placed in 1.5mL Eppendorf tubes, sealed with sealing film and transported on dry ice, and DNA can be transported in ice packs.
    • Sample preservation: cell samples or fresh tissue blocks are cut and stored at -80°C after freezing in liquid nitrogen. DNA samples can be stored at -20°C for a short period of time to avoid repeated freezing and thawing.

Applications of DNA Methylation Sequencing

  • Gene Expression Regulation
  • Tumor Research
  • Chromatin Remodeling
  • Genetic Imprinting
  • Disease Research
  • Embryonic Development
  • Environment and Epigenetics
  • Epigenetic Heterogeneity

References:

  1. Kretzmer H, Bernhart S H, Wang W, et al. (2015) "DNA methylome analysis in Burkitt and follicular lymphomas identifies differentially methylated regions linked to somatic mutation and transcriptional control." Nature genetics, 47(11): 1316-1325.
  2. Heller G, Topakian T, Altenberger C, et al. (2016) "Next-generation sequencing identifies major DNA methylation changesduring progression of Ph+ chronic myeloid leukemia." Leukemia, 30(9):1861-1868.
  3. Yan H, Bombarely A, Xu B, et al. (2018) "siRNAs regulate DNA methylation and interfere with gene and lncRNA expression in theheterozygous polyploid switchgrass." Biotechnology for Biofuels, 11(1):208.
* It should be noted that our service is only used for research, not for clinical use.

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