DNA / RNA Extraction

DNA / RNA Extraction Introduction

Genomic DNA and total RNA extracted from cells are mixed with proteins, polysaccharides, and nucleic acids of various sizes. The process of removing these "impurities" is the process of nucleic acid purification. During the isolation and purification of nucleic acids, in order to prevent the denaturation and degradation of nucleic acid macromolecules, it must be operated at a low temperature of 0 ~ 4℃. The nuclease hydrolysis is a serious obstacle in the past to prepare active nucleic acid macromolecules. Therefore, inhibition of nuclease activity is the main measure to ensure the integrity of nucleic acid macromolecules in the purification process.

DNA Extraction

DNA extraction is a method of obtaining DNA from samples such as cells. DNA extraction is divided into three basic steps:

  1. Cells are disrupted by grinding or sonication, and membrane lipids are removed by adding detergent.
  2. Add protease, acetate precipitation, or phenol / chloroform extraction to remove intracellular proteins, such as histones bound to DNA.
  3. Precipitate DNA in cold ethanol or isopropanol, because DNA is insoluble in alcohol and sticks together. This step can also remove salt.

DNA / RNA Extraction IntroductionFig. 1 Basic steps of all DNA extraction methods.

Different extraction methods have different DNA yields and purity. Common DNA extraction methods are: organic extraction, magnetic purification, ion exchange technology, salting out method, cesium chloride density gradient centrifugation method and Chelex 100 resin purification method. Different cell DNA, its extraction method often needs to be improved and optimized. Cetyltrimethylammonium bromide (CTAB) and isothiocyanate (GITC) are commonly used to extract plant tissue DNA.

RNA Extraction

RNA extraction is the purification of RNA from biological samples. The problem with this step is that the cells and tissues are full of ribonucleases, which quickly degrade RNA. There are several methods for molecular isolation of RNA from samples. One of the most commonly used methods is guanidine thiocyanate-phenol-chloroform extraction. In addition, using a mortar and pestle or a professional electric grinder to extract RNA from liquid nitrogen is also an effective method to prevent ribonuclease.

DNA / RNA Extraction Service

Creative Biogene can extract genomic DNA and total RNA from a variety of materials. Due to the different amounts of genomic DNA and total RNA extracted from different types of materials, even if the same material has different freshness, the difference is relatively large, so please provide fresh and sufficient experimental materials, and use specific methods to store and transport them. The services we can provide are as follows.

Genomic DNA Extraction and Purification Services

Service items Material requirements Amount of DNA
Bacterial genomic DNA extraction 1-2 mL of fresh bacterial solution in logarithmic growth phase 1-2 μg
Genomic DNA extraction from cells 1 × 106 fresh or frozen cells 10 μg
Blood Genomic DNA Extraction 1 mL fresh or liquid nitrogen frozen samples 10 μg
Tissue genomic DNA extraction 100 mg fresh sample or frozen sample at -20℃ 10 μg

Total RNA Extraction and Purification Services

Service items Material requirements Amount of DNA
Total RNA extraction from bacteria 1-2 mL of fresh bacterial solution in logarithmic growth phase 10 μg
Cell total RNA extraction 1 × 106 cells in suspension or freshly harvested 1 μg
Cell total RNA extraction 1 mL fresh or liquid nitrogen frozen samples 1 μg
Total RNA extraction from animal tissues 100 mg fresh or liquid nitrogen frozen samples 100 μg
Plant tissue total RNA extraction 100 mg fresh or liquid nitrogen frozen samples 10 μg

You can customize the DNA / RNA extraction service by phone or email, and our colleagues will reply your inquiry within three working days.

* It should be noted that our service is only used for research, not for clinical use.

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