A primer is a short single-stranded nucleic acid utilized by all living organisms in the initiation of DNA synthesis. The polymerase chain reaction (PCR) uses a pair of custom primers to direct DNA elongation toward each-other at opposite ends of the sequence being amplified. These primers are typically between 18 and 24 bases in length, and must code for only the specific upstream and downstream sites of the sequence being amplified. PCR primers can affect the results of a PCR reaction, an incorrect PCR primer can lead to a failed reaction- one in which the wrong gene fragment or no fragment is synthesized. Therefore, important considerations for primer design are melting temperature, annealing temperature, promoter region, and unique sequence targets.
Fig.1 Diagrammatic representation of the forward and reverse primers for a standard PCR
Creative Biogene can provide you with one-stop custom PCR primer design and synthesis service, aiming to save time, effort, and money from preventing complications or even failures in PCR experiments. Because PCR primer sets can greatly affect results, these designs must be optimized and tested before using primers. With years of expertise in PCR / qPCR research and formulation, the scientists of Creative Biogene are able to design PCR primers for sequence analysis, gene expression, genotyping, cloning, mutation detection, and clinical diagnosis.