DNA methylation refers to the process by which methyl groups on S-adenosylmethionine (SAM) are added to the bases of DNA molecules under the catalysis of methyltransferases. The covalent bond between the 5th carbon atom of cytosine and the methyl group is a common form of methylation, and cytosine is modified to 5-methylcytosine (5mC). DNA methylation is tissue-specific, dynamic, sequence context-dependent, and inherited across generations, and it is one of the most studied epigenetic modifications. Explore the significance of epigenetic research by studying the correlation of DNA methylation with tumors, neurodegenerative diseases, and autoimmune diseases, as well as processes such as embryonic development, genetic imprinting, and X chromosome inactivation.
Whole Genome Bisulfite Sequencing (WGBS) is regarded as the "gold standard" for DNA methylation research, combining bisulfite (Bisulfite) processing and high-throughput sequencing technology to achieve genome-wide scope The methylation analysis of a single C base is suitable for the construction of a genome-wide fine methylation map.
Methods for genome-wide DNA methylation analysis.
Our WGBS mainly converts unmethylated cytosine into uracil through bisulfite (Bisulfite), and then combines the Illumina high-throughput sequencing platform to measure the methylation level of cytosine in the whole genome. Perform assays to accurately analyze the methylation status of each cytosine, so as to construct a highly accurate genome-wide methylation map to help you explore the important mechanism of cytosine methylation in human diseases, cell growth and development.
| Service items | Material requirements |
| Tissue samples | Fresh tissue≥5 mg, immediately freeze in liquid nitrogen for half an hour. Store at -80°C and ship on dry ice. |
| DNA sample | DNA is dissolved in TE buffer, the total amount is≥300ng, the concentration is 20-100 ng/μL., and the volume is≥10 μL. |
| Blood sample | The total amount of plasma/serum≥2.5 mL, anticoagulation tube: sodium citrate tube or EDTA tube, heparin anticoagulation tube is not recommended. Store at -80°C and ship on dry ice. Total blood≥7.5 mL, anticoagulation tube: sodium citrate tube or EDTA tube, heparin anticoagulation tube is not recommended. Store at -80°C and ship on dry ice. |
| Paraffin embedded sample | Provide 10 tissue sections (area > 10 mm × 10 mm, thickness about 5-10 μm). FFPE slices are shipped at room temperature. |
You can customize the methylation-specific PCR service by phone or email, and our colleagues will reply your inquiry within three working days.
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