SNP genotyping is a measure of the genetic variation of single nucleotide polymorphism (SNP) among species. SNP is one of the most common types of genetic variation. SNP is a single base pair mutation at a specific site, usually consisting of two alleles (rare allele frequency > 1%). SNP is associated with many human diseases including rheumatoid arthritis, diabetes, and multiple sclerosis. It is particularly important in pharmacogenetics. Real time PCR is an effective method for SNP genotyping. It provides a variety of markers for the detection of genetic variation, including DNA insertioners such as SYBR green, fluorescent probes and widely used TaqMan chemical methods.
Fig. 1 Schematic representation of TaqMan SNP genotyping. (Chris B, et al. 2010)
SNP Genotyping Service
As a supplier dedicated to the production and research of PCR products, the research team of Creative Biogene has professional basic knowledge in the field of real-time PCR and SNP genotyping. Creative Biogene can provide you with professional SNP genotyping service using real-time PCR. The consequential analysis can also be carried out by our powerful bioinformatics platform.
The Workflow of SNP Genotyping Service
- Multiple sample types acceptance: we currently have wide range of kits for purifying genomic DNA from a variety of samples including DNA, cell and tissue samples for our SNP genotyping service.
- Strict quality control: we have strict quality control for each step of SNP genotyping to ensure that our customers receive quality data.
- One-stop service: you only need to provide samples. We provide comprehensive service from sample preparation to data analysis.
- High accuracy: it can accurately detect the degree percentage of single nucleotide polymorphisms (SNPs).
Requirements for Samples
||Concentration ≥ 20ng / μL, volume ≥ 20 μL
||concentration ≥ 20ng / μL, volume ≥ 20 μL
||≥ 500 μL anticoagulant; standard sampling tube is used, and EDTA is recommended for anticoagulant
||fresh (-80℃) or paraffin embedded tissue, fixed in 95% ethanol, with tissue amount ≥ 300mg
Delivery of SNP Genotyping Service
- Experimental flow
- Designed primer sequence
- Sequencing results
- SNP genotyping results
- Variant detection
- SNP Genotyping
- PCR data analysis
You can customize the SNP genotyping service by phone or email, and our colleagues will reply your inquiry within three working days.