Circular RNA (circRNA) is a special kind of noncoding RNA (sometimes expressed in vivo), which is the latest research hotspot in RNA field. Different from the traditional linear RNA (including 5' and 3' ends), the circRNA molecule has a closed ring structure, which is not affected by RNA exonuclease, more stable expression and is not easy to degrade. In terms of function, recent studies have shown that circRNA molecules are rich in microRNA binding sites, which play the role of miRNA sponge in cells, thus relieving the inhibition of miRNA on its target genes and increasing the expression level of target genes. This mechanism is called competitive endogenous RNA (ceRNA) mechanism. CircRNA plays an important regulatory role in diseases through the interaction of miRNAs associated with diseases. Study of circRNA can be achieved by real time PCR. (Fig. 1)
Fig. 1 Schematic representation of the design of divergent primers using the circRNA junction as template for PCR amplification. (Amaresh C, et al. 2018)
As a company dedicated to the production and research of PCR products, the research team of Creative Biogene has professional basic knowledge in the field of real-time PCR and circRNA expression analysis. Creative Biogene can use real-time PCR to provide you with professional circRNA expression analysis service. Through our powerful bioinformatics platform, we can provide you with comprehensive data analysis.
| Service items | Material requirements |
| Tissue sample | ≥ 100 mg, must be stored in RNA protection solution. |
| Blood sample | volume ≥ 2 mL |
| Total RNA | total RNA ≥ μg |
| Cell culture in vitro | ≥ 2 × 106 cells, must be stored in RNA protection solution. |
You can customize the circRNA expression analysis service by phone or email, and our colleagues will reply your inquiry within three working days.