CircRNA Expression Analysis

CircRNA Expression Analysis

Circular RNA (circRNA) is a special kind of noncoding RNA (sometimes expressed in vivo), which is the latest research hotspot in RNA field. Different from the traditional linear RNA (including 5' and 3' ends), the circRNA molecule has a closed ring structure, which is not affected by RNA exonuclease, more stable expression and is not easy to degrade. In terms of function, recent studies have shown that circRNA molecules are rich in microRNA binding sites, which play the role of miRNA sponge in cells, thus relieving the inhibition of miRNA on its target genes and increasing the expression level of target genes. This mechanism is called competitive endogenous RNA (ceRNA) mechanism. CircRNA plays an important regulatory role in diseases through the interaction of miRNAs associated with diseases. Study of circRNA can be achieved by real time PCR. (Fig. 1)

Schematic representation of the design of divergent primers using the circRNA junction as template for PCR amplification. Fig. 1 Schematic representation of the design of divergent primers using the circRNA junction as template for PCR amplification. (Amaresh C, et al. 2018)

CircRNA Expression Analysis Service

As a company dedicated to the production and research of PCR products, the research team of Creative Biogene has professional basic knowledge in the field of real-time PCR and circRNA expression analysis. Creative Biogene can use real-time PCR to provide you with professional circRNA expression analysis service. Through our powerful bioinformatics platform, we can provide you with comprehensive data analysis.

The Workflow of CircRNA Expression Analysis Service

The Workflow of CircRNA Expression Analysis Service

Our Advantages

  • Strict quality control: we have strict quality control for each step of circRNA expression analysis to ensure that our customers receive high quality data.
  • Excellent sample processing technology: processing various types of samples to eliminate linear RNA interference.
  • Comprehensive matching test: it can be matched with related linear RNA or miRNA qPCR test to provide you with comprehensive results.

Requirements for Samples

Service items Material requirements
Tissue sample ≥ 100 mg, must be stored in RNA protection solution.
Blood sample volume ≥ 2 mL
Total RNA total RNA ≥ μg
Cell culture in vitro  ≥ 2 × 106 cells, must be stored in RNA protection solution.

Delivery of CircRNA Expression Analysis Service

  • Provide raw data, amplification curve and fusion curve analysis
  • Related miRNA expression analysis
  • Comparison of linear RNA and circular RNA
  • circRNA expression analysis

You can customize the circRNA expression analysis service by phone or email, and our colleagues will reply your inquiry within three working days.

* It should be noted that our service is only used for research, not for clinical use.

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