Oligonucleotides Modifications

Oligonucleotides have a wide range of applications in the fields of biotechnology, molecular biology, diagnosis and therapy. Chemically synthesized oligonucleotides can be modified in their structure to improve the characteristics of oligonucleotides, including increasing stability, specificity, and affinity.

Oligonucleotides Modifications

Oligonucleotides Modifications

As a professional biotechnology company, Creative Biogene can provide you with a series of customized high-quality, low-cost oligonucleotide modification services. In order to meet the desired purity, we use high performance liquid chromatography (HPLC) to purify oligonucleotides that have been modified. If you have any needs, please feel free to contact us.

Our Services

We can customize specific oligonucleotide modification schemes according to your scientific research needs, such as the following schemes:

Location of Modifications Modification Types
Modification of oligonucleotides at the 5'-end Phosphorylation, amination, thiolation, introduction of a spacer, conjugation, branching phosphoramidites, blocking of 5'end with 5'-OMedT
Modifications of oligonucleotides at the 3'-end 3'-phosphate group, 3'-amino group, 3'-sulphydryl group, introduction of a spacer arm at the 3'-end, conjugation, 3'- 3' linked oligonucleotides, modified bases at the 3'-terminus
Modification of oligonucleotides at the internucleotide linkages phosphorothioates, methylphosphonates, phosphorodithioates, oligonucleotides with 3'-3' and 5'-5' linkages
Modification at the 2'-position 2'-O-alkyl-RNA, 2'-fluoro-U, 2'-fluoro-C, phosphorothioate-RNA
Modification at the ribose unit Deoxyuridine, 3'-Deoxyadenosine, dideoxyadenosine and dideoxycytosine, α-Oligonucleotides, ara cytidine
Modification at the nucleobases Inosine, deoxynaphtosine, etheno-adenosine and etheno-cytidine, C-5 modified deoxycytidine and deoxyuridine, introduction of a primary amino group by modified bases, introduction of a carboxy group by modified bases, introduction of biotin via modified bases, other modified bases, convertible nucleosides

Purification Options Offered

Purification method Description Benefit
Desalt Oligonucleotides were passed through a normal phase chromatography column which removed salts but not failed sequences. A salt-free DNA solution ready for immediate use; suitable for many PCR and sequencing applications without further purification.
Cartridge Based on reverse phase chromatography; removes failure sequences from the completed synthesis. Provides the full-length sequences required by some applications.
HPLC Reversed-phase high-performance liquid chromatography (HPLC) works the same way as purification columns, eliminating faulty sequences or unbound tags. Ensure that highly purified oligonucleotides are required in some applications.


Dry powder delivery, according to your needs of the separate packing.
COA files.

Our Advantages

  • Personalized customization: We can provide a variety of modification services according to your needs.
  • Strict quality control: The quality of our products is strictly controlled.
  • Quality analysis: We can provide a variety of analysis methods and related analysis files according to your needs.
  • Professional support: Our team can provide you with professional consultation and technical support.
  • Fast delivery: Flexible project arrangement shortens delivery time.
* It should be noted that our service is only used for research, not for clinical use.
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