LocNA Probe Services

Since Zamecnik and Stephenson first developed oligonucleotides as genetic-engineering tools in 1978, multiple DNA or RNA analogues have been introduced, such as peptide nucleic acid (PNA) and hexitol nucleic acid (HNA). Besides, another novel class of RNA derivatives, Locked Nucleic Acids (LNA or LocNA), was reported by the Wengel and Imanishi laboratories in 2001. Since the synthesis protocol of LocNAs is similar to that of DNA or RNA, LocNAs can be easily incorporated into kinds of qPCR detection chemistries, such as Dual-Labeled probes, Molecular Beacons and Scorpions Probes, to fine tune the properties of oligomers. Therefore, LocNA probes have been successfully served as a kind of powerful tools for biomedical research and detections.

Structures

As shown in Figure 1, compared with the conventional DNA monomer structure, there is an additional methylene bridge connecting the 2'-O and the 4'-C of the ribose ring in the LocNA monomer structure. Apparently, this bridge locks the conformation and limits the flexibility of the ribose. Consequently, the duplexes made by LocNA and other nucleic acids could be more stable.

LocNA Probe ServicesFigure 1. Structures of (A) Locked Nucleic Acid (LocNA) and (B) conventional DNA monomers.

Advantages

  • Due to the intermolecular bridges, LocNAs can always bind their target DNA sequences with higher thermal stabilities of 3-8℃ per base, allowing the designs of probes shorter than normal size probes.
  • LocNA probes have high specificity. Mutant alleles cannot be able to bind to LocNA probes, so wild-type allele amplifications will be suppressed.
  • LocNA probes are more accurate in allelic discrimination
  • Multiple quenchers and reporter dyes can be used in LcNA probes.
  • Small-scale is available, therefore it's quite suitable for lab research.

Application

Besides allele discrimination, there are also many applications of LocNA probes, such as detections for SNP and pathogen, viral load quantification, gene-related analysis and Multiplexing, etc. In addition, it has been reported that LocNA probes can also be used in antisense mRNA technology.

Our services

Our company can design and synthesize various LocNA probes. The details are shown below:

  • Multiple scales can be provided to meet different research purposes.
  • LocNA probes with different reporter dyes are available, such as FAM, TEX, YAK and HEX dyes, etc.
  • The probe products will be qualified by QC with MALDI-TOF MS and analytical HPLC.

References:

  1. Kauppinen S, Vester B, Wengel J. Locked nucleic acid: high-affinity targeting of complementary RNA for RNomics. Handbook of Experimental Pharmacology, 2006, 173(173): 405-422.
  2. Kauppinen S, Vester B, Wengel J. Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics. Drug Discovery TodayTechnologies, 2005, 2(3): 287-290.
  3. Vester B, Wengel J. LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA. Biochemistry, 2004, 43(42): 13233-13241.
* It should be noted that our service is only used for research, not for clinical use.

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