PCR Mutagenesis

Introduction of Mutagenesis

Mutagenesis is a fundamentally important DNA manipulation technique, including the addition, deletion, and point mutation of DNA molecular bases. It is mainly to study the effect of this change on the function of genes or DNA. In general, there are different mutation strategies for different research purposes, which can be divided into two types: site-directed mutation and random mutation. Among them, site directed mutation can effectively change the properties and characteristics of the target proteins and genes, which is one of the most essential techniques to study the structure-function relationship of genes and proteins.

PCR Mutagenesis

Polymerase chain reaction (PCR) technology has revolutionized the process of isolating and amplifying segments of DNA, which is a method for generating site-directed mutagenesis. PCR-based methods such as overlap extension, inverse PCR, and megaprimer PCR were developed to generate mutations (base substitutions, insertions, and deletions) from double-stranded plasmid without the need for subcloning into M13-based bacteriophage vectors and for ssDNA rescue.

PCR Mutagenesis Service

The proven expertise of Creative Biogene's team in the field of in vitro mutagenesis combined with our optimized technology allows us to deliver mutated and verified vectors (purified DNA and bacterial clones) within only a few days, thereby saving scientists' valuable time. Moreover, Creative Biogene can provide comprehensive upstream and downstream services of PCR mutagenesis, including template sequencing, expression vector construction, and protein expression and purification.

Fig.1 Site directed mutagenesis by PCR

The Workflow of PCR Mutagenesis

Our Advantages

• Unparalleled accuracy: our high performance PCR polymerase ensures accuracy of mutants, avoiding creation of unwanted mutations.
• Unlimited sites: with our sophisticated gene synthesis and mutagenesis techniques, we can introduce mutations at any site.
• Flexibility: generate substitutions, deletions, or insertions in virtually any plasmid.
• Optimal mutation scheme: the optimal mutation scheme can be designed and optimized according to the experimental requirements, so that the experimental operation is faster and more convenient.
• One-stop solutions: we offer comprehensive upstream and downstream services for PCR mutagenesis, including template DNA sequencing, gene synthesis,customized vector construction, and protein expression and purification.

Requirements for Samples

Delivery Specifications for Mutagenesis Service

Our mutagenesis service offers the following delivery package:

• 4 μg of lyophilized plasmid containing your gene insert (1 μg for low-copy plasmid).
• Sequence chromatograms covering your gene (electronic).
• Construct map for the plasmid (electronic).
• COA document.

If the specific site on your gene or vector is not what you need, or you need to do key control to carry out functional research on genes, components, etc., you may need to make point mutation on its specific location point, or make point mutation on multiple sites. You can customize the PCR mutagenesis service by phone or email, and our colleagues will reply your inquiry within three working days.