Sequencing

What Is Sequencing?

Sequencing refers to a method of determining the sequence of a gene and is a new genetic testing technology. The genes of each species control the expression of biological traits, and sequencing refers to the determination of the sequence of genes. It is able to analyze and measure the full sequence of genes from blood or saliva to predict the likelihood of developing a variety of diseases, behavioral traits and rational behavior of individuals. Genetic sequencing technology can target individual diseased genes for early prevention and treatment.

What Is Sequencing Figure 1. The process of next-generation sequencing.

Advances in Sequencing Technology

In the 1970s, Frederick Sanger invented the double deoxy chain termination method of nucleic acid sequencing (Sanger sequencing), also known as the first-generation sequencing technology. It is based on the principle of PCR amplification using a single strand of DNA as a template. The bases added to the amplification system are dNTP and fluorescent ddNTP, and the obtained products of different lengths are separated by electrophoresis and differentiated by laser-induced fluorescence color, and DNA strands up to 800bp long are obtained after information conversion. This method is widely used in sequencing PCR products and vector clones, but its drawbacks of high cost and low sequencing throughput limit the application of this method in large-scale sequencing.

Next-generation sequencing technology, also known as second-generation sequencing technology, is represented by Solexa from Illumina, SOLiD from ABI and 454 from Roche, and is best characterized by high throughput of data output.

In terms of years' professional experience in this field, Creative Biogene can provide you with the most affordable and highest quality sequencing services, ranging from Sanger sequencing to simple and scalable second-generation sequencing. We can provide a sequencing platform that meets your needs for fast, accurate and cost-effective results with optimized applications, high-quality consumables and simplified analytical solutions.

Applications of Sequencing

  • Oncology
  • Inherited Disease
  • Microbial
  • Human Identification
  • Reproductive Health Research

Technologies

  • Next-Generation Sequencing
    • Study of diseases with higher levels of phenotypic heterogeneity
    • Non-differential sequencing of large numbers of genes to discover novel mutations
    • Sequencing data up to 260 million reads per day
  • Sanger Sequencing
    • Study the list of genes associated with specific phenotypes
    • Confirm NGS variants
    • Detection of minor allele fragments down to 5%
    • Read contiguous sequences of up to 1000 bases
  • Fragment analysis
    • DNA fragmentation analysis enables a variety of applications from genotyping to bacterial identification, from plant screening to gene expression profiling.

References:

  1. Wong, LL, Razali, SA, Deris, ZM, et al.(2022) 'Application of second-generation sequencing (SGS) and third generation sequencing (TGS) in aquaculture breeding program.' AQUACULTURE, 548. doi: 10.1016/j.aquaculture.2021.737633
  2. Land, M., Hauser, L., Jun, S.R., et al. (2015) Insights from 20 years of bacterial genome sequencing. Functional & Integrative Genomics,15 (2). doi:10.1007/s10142-015-0433-4.
* It should be noted that our service is only used for research, not for clinical use.

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