Viral DNA & RNA Purification

Viruses are the simplest organisms whose genomes can be composed of either DNA or RNA. Each viral particle contains only one nucleic acid, either DNA or RNA, and the two generally do not coexist in the same viral particle. The DNA and RNA that make up the viral genome can be single-stranded or double-stranded, closed-loop molecules or linear molecules. In general, the genomes of most DNA viruses have double-stranded DNA molecules, while the genomes of most RNA viruses are single-stranded RNA molecules.

Virus analysis of biological and environmental samples requires rapid and efficient purification of high quality viral nucleic acids in a variety of sample types. And with nucleic acid testing, the presence of viral nucleic acids in the blood can be detected to diagnose the presence of pathogenic infections in the organism and shorten the detection window of viruses, thus improving diagnosis and cure rates.

In terms of years' professional experience in this field, Creative Biogene can provide you with viral DNA & RNA purification.

Viral DNA & RNA PurificationFigure 1. The process of viral DNA&RNA purification

Our Viral DNA & RNA Purification Methods

Virus analysis of biological and environmental samples requires rapid and efficient purification of high quality viral nucleic acids in a variety of sample types. Creative Biogene can provide a variety of methods for purifying viral DNA/RNA.

Magnetic bead-based viral RNA/DNA purification with a rapid process of 96 samples in <40 minutes, flexible protocols for a wide range of sample types including blood, urine, swabs, lavage and various delivery media (e.g. universal viral delivery cultures), sample starting volumes of 200-2 mL, and compatibility with bronchoalveolar lavage and other respiratory samples. The sample starting volume is 200-2 mL, compatible with bronchoalveolar lavage and other respiratory samples.

Centrifugal column viral RNA/DNA purification for rapid and easy isolation of viral RNA or DNA from cell-free samples, allowing high concentrations of viral nucleic acids for greater detection sensitivity, ideal for real-time PCR and endpoint analysis.

References:

  1. Shigeki M , Kayoko F , Chieko M , et al. High-throughput HBV DNA and HCV RNA detection system using a nucleic acid purification robot and real-time detection PCR: its application to analysis of posttransfusion hepatitis. Transfusion, 2019(42):100-106.
  2. Ta D T , Chu K L , Soonaan N , et al. A New and Simplified Anion Exchange Chromatographic Process for The Purification of Cell-Grown Influenza A H1N1 Virus. Separation and Purification Technology, 2021, 263(Suppl 2):118412.
  3. Poirier E Z , Bertsy G , Lorena T P , et al. Dicer-2-Dependent Generation of Viral DNA from Defective Genomes of RNA Viruses Modulates Antiviral Immunity in Insects. Cell host & microbe, 2021(23):353-365.
* It should be noted that our service is only used for research, not for clinical use.

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