Custom Probes Services

A probe is a nucleic acid molecule that can specifically hybridize with a certain target sequence according to the Watson-Crick complementary condition. According to different lengths, there are generally two classes of probes, gene probes with more than 500 bases and oligonucleotide probes with the length of about 18-30 bases. For the longer probes, the production of an unknown DNA sequence or a specific clone in need can be achieved by clone probes, while polymerase chain reaction (PCR) is actually the most extensively used method due to the high efficiency and high production.

As for oligonucleotide probes, there are four different types, including DNA probes, RNA probes, cDNA probes and synthetic oligonucleotide probes. Basically, an oligonucleotide probe should be identified, reproduced in sufficient quantity and perfectly bind to its target sequence with strong affinity. Although the length of 18-30 bases is common, scientists are now able to synthesize oligonucleotide probes with up to 100 bases.

Creative Biogene provides customers with a series of services from probe design to finished products.

  • Multiple types and scales of probes are available;
  • A highly efficient and cost-effective synthesis will be provided;
  • All the RNA products will be determined by using MALDI-TOF, HPLC purification, Electrospray Ionization (ESI) Mass Spec and OD by UV spectroscopy.
  • Shipments include a quality assurance document for your records.

Please check the following pages for accessing more detailed product information.

References:

  1. Wang D X, Wang J, Wang Y X, et al. DNA nanostructure-based nucleic acid probes: construction and biological applications. Chemical Science, 2021, 12(22): 7602-7622.
  2. Vizzini P, Iacumin L, Comi G, et al. Development and application of DNA molecular probes. Journal of Bioengineering, 2017, 113-132.
  3. Rio, D. C., et al. RNA: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 2011, 77-87.
  4. Cooper, Geoffery M. The Cell: A Molecular Approach. 4th ed. Washington D.C.: ASM Press, 2007, 131-2.
  5. Hua R, Yu S, Liu M, Li H. A PCR-based method for RNA probes and applications in neuroscience. Frontiers in Neuroscience, 2018, 12, 266.
* It should be noted that our service is only used for research, not for clinical use.

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