Regarding the mechanism of the polymerase chain reaction (PCR) technique, a probe usually represents a fragment of DNA or RNA functioning by hybridization with target double-stranded DNA, in which a primer can initialize this progress by hybridizing with a specific single-stranded DNA. Therefore, besides the application of qPCR, probe-based detection has also been used extensively in many biochemical or biomedical fields, such as the detections of pathogenic microorganisms, changes to nucleic acid sequences and tandem repeat sequences, etc.
Generally speaking, several common factors should be considered carefully for both the probe and primer when running successful PCR progress: the location, melting temperature (Tm), Annealing temperature (Ta) and GC content (35-65%). While the Tm and Ta of probes usually are 6-8 °C higher and 5°C lower than those of primers, respectively. For more detailed information, please check the corresponding pages.
Creative Biogene has been committed to providing high-quality custom probes and primers. We also support one-stop services from design to product shipment.