Custom Primers & Probes

Regarding the mechanism of the polymerase chain reaction (PCR) technique, a probe usually represents a fragment of DNA or RNA functioning by hybridization with target double-stranded DNA, in which a primer can initialize this progress by hybridizing with a specific single-stranded DNA. Therefore, besides the application of qPCR, probe-based detection has also been used extensively in many biochemical or biomedical fields, such as the detections of pathogenic microorganisms, changes to nucleic acid sequences and tandem repeat sequences, etc.

Generally speaking, several common factors should be considered carefully for both the probe and primer when running successful PCR progress: the location, melting temperature (Tm), Annealing temperature (Ta) and GC content (35-65%). While the Tm and Ta of probes usually are 6-8 °C higher and 5°C lower than those of primers, respectively. For more detailed information, please check the corresponding pages.

Creative Biogene has been committed to providing high-quality custom probes and primers. We also support one-stop services from design to product shipment.

  • Multiple types and scales of probes are available;
  • A highly efficient and cost-effective synthesis will be provided;
  • All products will be determined by using MALDI-TOF, HPLC purification, Electrospray Ionization (ESI) Mass Spec and OD by UV spectroscopy.
  • Shipments include a quality assurance document for your records.

References:

  1. Thornton B, Basu C. Rapid and simple method of qPCR primer design. Methods In Molecular Biology, 2015, 1275: 173-179.
  2. Dieffenbach C W, Lowe T M, Dveksler G S. General concepts for PCR primer design. PCR Methods and Applications, 1993, 3(3): S30-S37.
  3. Vizzini P, Iacumin L, Comi G, et al. Development and application of DNA molecular probes. Journal of Bioengineering, 2017, 113-132.
  4. Cooper, Geoffery M. The Cell: A Molecular Approach. 4th ed. Washington D.C.: ASM Press, 2007, 131-2.
  5. Hua R, Yu S, Liu M, Li H. A PCR-based method for RNA probes and applications in neuroscience. Frontiers in Neuroscience, 2018, 12, 266.
* It should be noted that our service is only used for research, not for clinical use.

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