Product Name
General RNA Extraction Kit
Product Description
This kit provides protocols to isolate total RNA from a wide range of sample types, including animal tissues, plant tissues, bacterial, yeast and mammalian cells, viruses, blood, plasma and serum. The first part of procedure is based on the work by Chomczynski and Sacchi ( 1987), namely, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt capable of protecting the RNA by inactivating endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based-membrane to which the RNA binds. Impurities and DNA do not bind to the column, and are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-free water and is suitable for use in a variety of downstream applications.
Components
Solution RL:60 mL
Wash Buffer RPI:18 mL
Wash Buffer RW:12 mL
DEPC-treated water:10 mL
RNase-free spin columns:50 each
RNase-free microcentrifuge Collection Tubes:50 each
Advantage / Highlights
•Consistent and high yields
•High-quality purified total RNA for downstream applications
Applications
The isolated RNA using this procedure can b used in:
Real-time reverse transcriptase PCR (RT-PCR)
Reverse transcriptase end-point PCR
Northern blotting
Nuclease protection assays
RNA amplification for microarray analysis
cDNA library preparation after poly(A)+ selection
Isothermal RNA amplification
RNAseq
Storage & Stability
Store at 2-8℃, protect from light. Kit contents are stable for up to 12 months, when properly stored.
