Product Name
HiScript II One Step qRT-PCR Probe Kit
Product Description
The detection rate of low copy number templates or weakly positive samples remains one of the challenges of clinical diagnosis. HiScript II One Step qRT-PCR Probe Kit contains the optimal ratio of HiScript II Reverse Transcriptase and Champagne Taq DNA Polymerase, and a fully optimized buffer system, whether total RNA, in vitro transcribed RNA, or viral RNA, HiScript II The One Step qRT-PCR Probe Kit has extremely high sensitivity, providing stable and reliable amplification performance. Multiple optimizations are possible without much optimization.
Components
RNase free ddH2O: 1.25 mL × 2
2 × One Step Probe Mix: 1.25 mL × 2
One Step SYBR Green Enzyme Mix: 250 μL
50 × ROX Reference Dye 1: 100 μL
50 × ROX Reference Dye 2: 100 μL
2 × One Step Probe Mix contains dNTP Mix, Mg 2+.
One Step SYBR Green Enzyme Mix contains HiScript Ⅱ Reverse Transcriptase, RNase inhibitor and Champagne Taq DNA Polymerase.
50 × ROX Reference Dye is used to correct the fluorescence signal error between wells.
Advantage / Highlights
• High detection sensitivity: Single-digit copies of templates or 0.1 pg of total RNA can be detected
• Multiple detection: support multiple probe detection
Applications
• This product is suitable for probe-based qRT-PCR using RNA as a template
Storage & Stability
Store at-20 ℃ in the dark
