Product Name
CDFast Probe RT-PCR + ROX Vial Kit (2000)
Product Description
CDFast Probe RT-PCR Kits deliver fast and sensitive real-time one-step RT-PCR quantification of RNA targets using probe-based detection. Q-bond technology and an optimized master mix enable shorter real-time RT-PCR run times, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. The kits are also supplied with an optimized RT mix for efficient cDNA synthesis in only 10 minutes. Two kit formats are available: the CDFast Probe RT-PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the CDFast Probe RT-PCR +ROX Vial Kit for all other cyclers. For convenience, the master mix can be stored at 2-8 °C.
Components
For 2000 × 25 µL reactions: 25 mL 2 × CDFast Probe RT-PCR Master Mix (without ROX dye), 1.05 mL ROX Dye Solution, 0.5 mL CDFast RT Mix, 20 mL RNase-Free Water
Advantage / Highlights
• For fast, one-step qRT-PCR using sequence-specific probes for gene expression analysis
• Faster results with time savings of up to 60%
• Sensitive detection of even low copy numbers
• Accurate detection of a wide range of template amounts
• Optimized, ready-to-use master mix for fast cycling
• One cycling protocol for all standard and fast cyclers
Applications
CDFast Probe RT-PCR Kits can be used for probe-based gene expression analysis of RNA targets on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent. For the CD-Gene Q and other CD-Gene cyclers, we recommend using the CD-Gene Probe RT-PCR Kit, which has been specially developed for fast cycling on these instruments.
