Product Name
CD Cell Multiplex Kits
Product Description
CD Cell Multiplex Kits accelerate and streamLine real-time RT-PCR and are ideal for experiments requiring rapid high-throughput gene expression analysis. The kits provide a set of buffers for preparing FastLane lysates from cultured cells. In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA. FastLane lysates are used directly in multiplex, real-time one-step RT-PCR (with or without ROX passive reference dye). A variety of other detection formats are also available, including detection with SYBR Green or sequence-specific probes.
Components
FastLane Cell One-Step Buffer Set, 2 × CDTect Multiplex RT-PCR Master Mix (contains ROX dye), and CDTect Multiplex RT Mix
Advantage / Highlights
• For multiplex, real-time one-step RT-PCR analysis from cultured cells without RNA purification
• No RNA purification required, significantly saving time
• Just 3 steps from cells to real-time RT-PCR
• High-throughput analysis of 96- and 384-well plates
• Unique gDNA Wipeout Buffer enables detection of RNA only
• Immediate startup using optimized reagents and protocols
Applications
CD Cell Multiplex Kits are ideal for experiments that require rapid, high-throughput gene expression analysis, such as:
Validation of siRNA-mediated gene knockdown
Screening of potential drugs
Detection of stimulated gene regulation
