Product Name
Omniscript RT Kit (200)
Product Description
The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low-copy numbers. The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. Simply add primers for fast and easy cDNA synthesis.
Components
For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 × 150 µL 10 × Buffer RT, 4 × 100 µL dNTP Mix (contains 5 mM each dNTP), 4 × 1.1 mL RNase-free water
Advantage / Highlights
• For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR
• High cDNA yields due to high-affinity enzyme
• Sensitive detection of as few as 10 copies of template
• No additional RNase H digestion step required
• Fast and easy procedure with no tedious pipetting steps
Applications
Omniscript Reverse Transcriptase is suitable for use in the following applications:
cDNA synthesis for end-point PCR
Synthesis of double-stranded cDNA for cloning
RACE (Rapid Amplification of cDNA Ends)
Linear RNA amplification
Exponential RNA amplification
SAGE (Serial Analysis of Gene Expression)
Labeling for microarrays
Analysis of transcription start site by primer extension
