Product Name
One Step RT PCR Kit (100 r × n / 50 µL)
Product Description
CD One Step RT-PCR Kit provides an easy and efficient way to perform both reverse transcription of RNA and PCR amplification of cDNA in one step. Only RNA template, primers and PCR-grade water are added.
The 20 × RT-RI Blend, which contains a blend of an efficient thermostable reverse transcriptase and a proprietary Ribonuclease Inhibitor, ensures high yields of cDNA.
The 2 × One Step Mix contains unique Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers in an optimized buffer and provides high PCR product yields with minimal background even when using low-abundance and difficult templates. PCR enhancers included in the mix allow efficient amplification of complex templates including GC or AT-rich sequences.
Components
2 × 1.25 mL One Step Mix
2 × 125 µL RT-RI Blend
Advantage / Highlights
• Efficient thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields
• Unique Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs
• PCR enhancers allowing sensitive low background amplification
Applications
• One-step RT-PCR
• Virus detection
• Amplification of GC-rich and complex templates
Storage & Stability
−20°C (until expiry date - see product label)