Pfu DNA Polymerase, 2.5 U / µL (100 U | 80 r × n)
Product name
Pfu DNA Polymerase, 2.5 U / µL (100 U | 80 r × n)
Product Name
Pfu DNA Polymerase, 2.5 U / µL (100 U | 80 r × n)
Cat No.
Product Description
CD Pfu DNA Polymerase is a highly purified thermostable recombinant proofreading DNA polymerase. Pfu DNA Polymerase exhibits approximately 10 times higher accuracy than Taq DNA polymerase and amplifies targets up to 3-4 kb in size. The enzyme catalyzes template-dependent nucleotide polymerization in the 5'→3' direction. Additionally the 3'→5' exonuclease (proofreading) activity corrects nucleotide incorporation errors, thereby increasing fidelity and accuracy of DNA polymerization. The enzyme has no 5'→3' exonuclease activity and no detectable reverse transcriptase activity and produces blunt-end PCR products. For the most demanding applications, the supplied 5 × PCR Enhancer can be optionally used for improving results when using templates with GC-rich sequences and complex structures.
40 µL Pfu DNA Polymerase 400 µL 10× Pfu Reaction Buffer 1.5 mL 5 × PCR Enhancer
Unit Definition
One unit catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction in 30 minutes at 72°C.
Advantage / Highlights
• Accurate PCR for demanding applications
• Approximately ten times higher accuracy than Taq DNA Polymerase
• Proof-reading for increased fidelity
• High-fidelity PCR
• Generation of PCR products for blunt cloning
• Site directed mutagenesis
Storage & Stability
−20°C (until expiry date - see product label)
* It should be noted that our products are only used for research, not for clinical use.
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