CD HS Super-Fidelity DNA Polymerase (100 U)
Product name
CD HS Super-Fidelity DNA Polymerase (100 U)
Cat.No.
PCR-0139
Size
Price
Product Name
CD HS Super-Fidelity DNA Polymerase (100 U)
Cat No.
PCR-0139
Price
Inquiry
Product Description
CD HS Super-Fidelity DNA Polymerase is a hot-start version of CD Super-Fidelity DNA Polymerase, which is based on the original addition and can inhibit 5'→ 3' polymerase activity and 3'→ 5' exonuclease activity at room temperature. The two monoclonal antibodies can be used for highly specific Hot Start PCR. 5 × CD SF reaction buffer has good adaptability for simple or complex template, short or long PCR amplification. The buffer already contains 10 mM Mg2+ (1 × final concentration of 2 mM). The reaction system can be optimized using DMSO or MgSO4 provided with the enzyme. The included PCR Enhancer facilitates the amplification of high GC content fragments.
Components
5 × SF Buffer (with 10 mM MgSO4) 100 μL 25 mM MgSO4) 1 mL dNTP Mix (10 mM each) 100 µL CD HS Super-Fidelity DNA Polymerase (1 U / μL) 100 µL 5 × PCR Enhancer 500 µL DMSO 100 µL 10 × Loading buffer 1.25 mL
Advantage / Highlights
•Added monoclonal antibodies for highly specific hot-start PCR
•Fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase
•Amplification speed is 4-150 times that of conventional polymerase
•The optimized buffer system is widely applicable to various templates
•Provides PCR Enhancer to help amplify high GC content templates
Applications
CD HS Super-Fidelity DNA Polymerase can be used for highly specific Hot Start PCR
Storage & Stability
Store at -20 °C
* It should be noted that our products are only used for research, not for clinical use.
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