CD PCR Cloningplus Kit (40)
Product name
CD PCR Cloningplus Kit (40)
Cat.No.
PCR-0117
Size
40
Price
Product Name
CD PCR Cloningplus Kit (40)
Cat No.
PCR-0117
Price
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Product Description
The CD PCR Cloningplus Kit provides ready-to-use ligation reactions, which contain linearized cloning vectors that carry U overhangs at each 3' end, allowing PCR products containing 3' end A overhangs to be directly ligated and cloned with high efficiency and speed. The CD PCR Cloningplus Kit also provides competent E coli. cells and SOC medium for efficient transformation.
Components
For 40 reactions: 2 × Ligation Master Mix (200 µL), pDrive Cloning Vector (2.0 µg), Distilled water (1.7 mL), CD EZ Competent Cells (40 tubes, 50 µL each), SOC medium (6 × 1.9 mL)
Advantage / Highlights
For direct cloning of PCR products Just 40 minutes from PCR product to plated cells Ready-to-use Ligation Master Mix High-specificity UA hybridization for efficient cloning Competent cells supplied with the kit Immediate plating of transformed competent cells
Applications
The CD PCR Cloningplus Kit is suitable for cloning of any PCR product that has a single A overhang at each 3' end. PCR products generated using Taq and other nonproofreading DNA polymerases can be directly cloned without any preparation. Kits offering proofreading DNA polymerases, such as the HotStar HiFidelity Polymerase Kit and the CD LongRange PCR Kit, generate PCR products with A overhangs, and are also highly suited for direct use with the CD PCR Cloningplus Kit.
* It should be noted that our products are only used for research, not for clinical use.
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