Product Description
HotStarTaq Master Mix contains HotStarTaq DNA Polymerase, the unique CD PCR Buffer that minimizes the requirement for optimization, and dNTPs. Providing all components in a master mix reduces pipetting steps and the risk of contamination, while increasing throughput and reproducibility.
Components
1 × 25 mL HotStarTaq Master Mix (contains 2500 units HotStarTaq DNA Polymerase PCR Buffer with 3 mM MgCl2, and 400 µM of each dNTP) and 1 × 50 mL RNase-Free Water
Advantage / Highlights
For highly specific amplification for any PCR application
High PCR specificity without the need for optimization
Easy reaction setup at room temperature
Ready-to-use master mix format reduces pipetting steps
Applications
HotStarTaq Master Mix Kit is highly suitable for a wide variety of applications, including challenging applications such as amplification of:
Complex genomic templates
Complex cDNA templates (e.g., RT-PCR)
Very low-copy targets (e.g., single-cell PCR)
Reactions with multiple primer pairs